基本信息

产品名称Recombinant Human EGF Protein英文名称Recombinant Human EGF Protein规格或纯度GMP, ≥95% (SDS-PAGE), Active, Pichia pastoris, No tag, 971-1023aa生物活性Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.内毒素水平≤ 1.0 EU/mg表达系统Pichia pastoris种属HumanAmino Acids971-1023aa序列NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR蛋白标签No tag蛋白长度Full length proteinCarrier FreeYesAnimal FreeYesAccession#P01133来源Recombinant预测分子量6.5kDSDS-PAGE7.1 kDa, under reducing conditions.

相关属性

特点与好处Application: Cell culture, FuncS, HPLC, SDS-PAGE物理外观Lyophilized复溶建议用无菌水重溶rhEGF冻干粉至100-200μg/ml，可用其他溶剂进行进一步稀释。溶解后的rhEGF 4℃可保存2-7天，尽快用完。若短期不用，请置于-20℃保存。 开启后，请尽快使用，避免污染。储存温度-20°C储存运输条件超低温冰袋运输稳定性与储存Upon delivery aliquot. Store at -20°C . Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.CAS编号和信息62253-63-8

常规信息

基因名EGF基因 ID1950细胞定位Membrane.序列相似性Contains 9 EGF-like domains. Contains 9 LDL-receptor class B repeats.涉及疾病Hypomagnesemia 4英文别名Recombinant Human Epidermal Growth Factor；rhEGF；beta-urogastrone；EGF；epidermal growth factor (beta-urogastrone)；epidermal growth factor；hEGF；HOMG4； pro-epidermal growth factor；URG；Urogastrone别名重组人表皮生长因子；Recombinant Human Epidermal Growth Factor； rhEGF；beta-urogastrone；EGF；epidermal growth factor (beta-urogastrone)；epidermal growth factor； hEGF； HOMG4; pro-epidermal growth factor；URG；Urogastrone

一般描述

pH值：6.5-7.5

人表皮生长因子（EGF）分子量为6.5kD，含有53个氨基酸，分子内有3个二硫键。EGF与位于细胞表面的特异受体EGFR 结合后，引起细胞内一系列的生化反应：提高细胞内的钙离子浓度水平，促进糖酵解的进行，增加蛋白质的合成，并且可以增强EGFR这一类特定基因的表达来促进DNA 的合成和细胞的增殖。

Purity

≥95% SDS-PAGE

Additional sequence information

Mature chain

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Post-translational

O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).

pH value: 6.5-7.5

Human epidermal cell growth factor, Mannitol, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Water

图片

Protein Bioactivity: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-Protein Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1ng/mL.

SDS-PAGE: Recombinant Human EGF Protein (rp155969)

Recombinant Human EGF Protein (rp155969)-SDS-PAGE

5μg/lane of Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 7.1 kDa.

安全信息

信号词Warning象形图 ghs07危险声明H315,H319,H335预防措施声明P261,P305+P351+P338,P280,P302+P352,P321,P405,P501,P264,P271,P304+P340,P403+P233,P362+P364,P264+P265,P337+P317,P332+P317,P319WGK Germany3